Nuclear grade graphite is a kind of key material in the high temperature gas-cooled reactor pebble-bed module (HTR-PM), where nuclear grade graphite acts as the fuel element matrix material, structural material and neutron reflector. In the reactor, the service environment of nuclear grade graphite suffers high temperature and strong neutron radiation. Both neutron radiation and the oxidation by the oxidizing impurities in HTGR coolant can cause the structure to damage and the properties to deteriorate. Therefore, it is of great significance to study the evolution of defects in nuclear grade graphite for improving the reactor safety. The effects of ion irradiation and oxidation on the point defects in IG-110 graphite are studied in this work. The 190 keV He⁺ implantation treatments at room temperature with fluences of 1 × 10¹⁵, 5 × 10¹⁵, 1 × 10¹⁶ and 1 × 10¹⁷ cm^–2 are performed to induce 0.029, 0.14, 0.29 and 2.9 displacements per atom respectively. Oxidation treatments are performed at 850 ℃ for 10, 15, 20 and 25 min. Different sequences of He⁺ ion irradiation and oxidation are performed, which include irradiation only (Irr.), oxidation only (Ox.), irradiation followed by oxidation (Irr.-Ox.), and oxidation followed by irradiation (Ox.-Irr.). Raman spectrum shows that with the increase of ion irradiation dose, the intensity ratio of D peak to G peak (I_D/I_G) first increases and then decreases, implying that the point defects in graphite are induced by ion irradiation and the point defects evolve as dose increases; the degree of graphitization increases after oxidation, implying that the point defects are recovered by the annealing effect at high temperature, and the point defects decrease after oxidation. This makes Ox.-Irr. samples have a lower point defect content than Irr. samples, and leads Irr.-Ox. samples to possess a higher point defect content than Ox. samples. The positron annihilation Doppler broadening tests reveal that there are only point defects after ion irradiation and oxidation have partially recovered point defects. The ion irradiation and oxidation have opposite effects on the evolution of point defect in graphite. The ion irradiation increases the average S-parameter and reduces the average W-parameter, while oxidation reduces the average S-parameter and increases the average W-parameter. The annealing effect at 850 ℃ cannot completely recover the point defects in Irr.-Ox. samples.